How to remove cloning vector sequence from bacterial assembled genome?
Hi All I have a bacterial genome assembly that contains contamination from plasmid vector sequences. How can I remove the contaminants from the assembly file?"
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GenoMax
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mathavanbioinfo
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mathavanbioinfo
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You could use an alignment program and vector reference to identify the vector (if it was assembled as a single piece in your data).
Ideally you need to start over. Clean the reads of vector contamination first followed by redoing the assembly.