Alternative To interactions comparison in limma in DESeq2
Is there an alternative to the make interactions comparison you are able to do in limma in DESeq2? i.e.
cont.matrix <- makeContrasts(
+ SvsUinWT=WT.S-WT.U,
+ SvsUinMu=Mu.S-Mu.U,
+ Diff=(Mu.S-Mu.U)-(WT.S-WT.U),
+ levels=design)
So I'm interested in getting the DESeq2 equivalent of the Diff = (Mu.S-Mu.U)-(WT.S-WT.U) comparison. Is this possible?
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https://support.bioconductor.org/p/9143932/ -- see the lower parts where Michael Love answers, it comes down to numeric contrasts.