Hi, Myself Abhirami, Currently pursuing Ph.D., for a paper publication currently I'm working on mitogenomic sequences.

So as the initial steps, I proceed successfully with fastp (Quality checking), cutadapt, bwa mem (to remove host genome).

But, while running megahit, I reached up with many difficulties of not generating results even though the generated output file seems to be empty with zero bytes.

Can you please address the solution for the issue with which I'm facing right now? I'm here mentioning the code for megahit with which I used (ubuntu 18.04) :

megahit --min-count 2 --k-min 27 --k-max 87 --k-step 10 --min-contig-len 100 -1 input_1.fastq -2 input_2.fastq -o megahit_assembly