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How to pass from DNA to AA fasta

Hi,

I hope you are well. I am trying to figure out how can I pass a eukaryotic gene that has introns to aa sequence. The only way that I have thought is to transcribe all the gene then translate it with the ORF that is correct, and then do a blast for proteins. With the highest identity% entry make an alignment and then cut the parts that do not have in common.

Any other idea, please?

Thank you for your help and time.

genetics
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updated by Buffo Buffo • written by Anderson Stiward • Anderson Stiward

What do you mean by "pass" in the context of a gene?

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written by Ram Ram

Hi i mean to convert it from DNA to AA, to do an in silico translation

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written by Anderson Stiward • Anderson Stiward

You should have just said "translate to AA" then, "pass" doesn't really mean anything.

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written by Ram Ram

If you know the intron sequence, remove it and use ORFfinder

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written by Buffo Buffo

Do you know another alternative If I do not have the sequence of the intron?

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written by Anderson Stiward • Anderson Stiward

If it is only one gene, maybe you can try to find it manually using the GT/AG universal intron motifs.

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written by Buffo Buffo

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