Hi all,
i am new to this single cell RNA-seq data analysis.
i've done cell type identification of whole brain mouse sample scRNA-seq data.
the results show that all neuron clusters are one-tenth less than glia clusters.
why is that happening? isn't that the neuron ratio almost equals to glia in the brain?
is that just coincidence from single cell library prep?
I appreciate your answers.
1 answer
Neurons are notoriously difficult to capture in scRNA-seq versus snRNA-seq for droplet based methods (i.e. 10X) because of cell morphology and durability concerns. Some of the newer microfluidics-free methods (like PIP-seq or split-pool combinatorial barcoding) tend to have an easier time capturing neurons in single-cell preps.
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