TRcaller is online for testing

Free account and testing is opening now

The best tandem repeat (TR) genotyping software for given loci from NGS data

the highest accuracy (99%) and fastest speed in seconds

For any short and long Next Generation Sequencing reads

Tested for forensic core 20 forensic core STRs, 38 XY STRs and 61 known disease-causing TRs in human

The free testing account is opened at www.trcaller.com

Abstract of the coming paper:

Calling tandem repeat (TR) variants from DNA sequences is of both theoretical and practical significance. Some bioinformatics tools have been developed for detecting or genotyping TRs. However, little study has been done to genotyping TR alleles from long-read sequencing data, and the accuracy of genotyping TR alleles from next generation sequencing data still needs to be improved. Herein, a novel algorithm is described to retrieve TR regions from sequence alignment, and a software program TRcaller has been developed and integrated into a web portal to call TR alleles from both short- and long-read sequences, both whole genome and targeted sequences generated from multiple sequencing platforms. All TR alleles are genotyped as haplotypes and the robust alleles will be reported, even multiple alleles in a DNA mixture. TRcaller could provide substantially higher accuracy (> 99% in 289 human individuals) in detecting TR alleles with magnitudes faster (e.g., ~2 seconds for 300x human sequence data) than the mainstream software tools. The web portal preselected 119 TR loci from forensics and disease plus customer giving TR loci. TRcaller is validated to be scalable in various applications, such as DNA forensics and disease diagnosis, which can be expanded into other fields like breeding programs.

Availability: TRcaller is available at www.trcaller.com