ArchR Doublet Inference to Signac
So I'm currently running Signac on an scATAC-seq dataset and have heard of individuals taking the doublet information from ArchR and sending it as a path to Signac. I can't find more information concerning this; any pointers?
• 1,632 views
•
link
1 answer
What they probably do is to run ArchR for quality assessment and cell filtering, and then they load the remaining cells into signac. \
Signac (like seurat) does not include any doublet inference tool, while ArchR does: addDoubletScores + filterDoublets
This is how the doublet info is stored inside and ArchR project:
>exp_Integrated@cellColData[,c("DoubletScore","DoubletEnrichment")]
DataFrame with 11477 rows and 2 columns
DoubletScore DoubletEnrichment
<array> <array>
WT#TAGGAGGCAAGGAACC-1 23.423640545554 2.9
WT#GTCGTAAAGATTAGAC-1 11.2245245084216 2.33333333333333
WT#ACGTGGCAGTGGTGTG-1 7.36953484802665 2.1
WT#AGCTGTAGTTTGTCTT-1 16.7685480948753 2.6
...
You can extract the list of UMIs that pass the filtering and use it to load the data in signac.
• 0 views
•
link
Log in to answer this question.