Hello! I have a question related to the evaluation of gene expression.
"2^-ΔΔCt" should be calculated for each sample or should the average value be used?
Method 1 - The average value of "ΔΔCt" for 5 samples and from this average value "2^-ΔΔCt" is calculated.
Method 2 - No average values for 5 samples. "ΔΔCt" is calculated for each of the 5 samples and "2^-ΔΔCt" is calculated for each of the 5 samples.
1 answer
Hi,
I believe you messaged me on ResearchGate. You have followed my post, here: How to report and plot qPCR data - delta delta Ct
You already have 3 replicates per Animal and condition (Control Treatment); so, I think that you should be calculating the 2^-ΔΔCt separately per animal. If you wish, after this, you then then report the average 2^-ΔΔCt across all animals.
Kevin
I think that the assumptions are still okay. Normally, our 'control' would just be 'healthy' human genomic DNA that is purchased by the lab; however, in your case, you have control samples. The Animals do not have to match across the Control and Treatment groups.
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