Renormalize after row/column deletion from TPM data in RNA-seq
I am working with single-cell data and received the TPM-normalized RNA-seq dataset from my labmate. Once I remove genes or cells during preprocessing, I notice that the data is no longer normalized (colSums from R is not returning 1e06). Is there any workaround to take care of this issue? I want to be able to perform downstream preprocessing with log(TPM+1) data.
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