Using DESeq2 to data normalization
Hello,
I have a single question in DESeq2 usage. I have multiple groups, but each has a single sample. Therefore, I could not use DESeq2 to obtain the fold change. Alternatively, I have a plan to do GSEA, but GSEA requested a normalized data as an input.
In that case, could I use DESeq2 only for normalisation? It looked ok but I want to make it sure.
Thank you very much.
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Yes, you can technically use DESeq2 as it performs normalization to adjust for differences in sequencing depth between samples.
Thank you very much.