The length of spacer between the promoter and ORF
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16 months ago
sunyeping ▴ 110

Dear all,

I am trying to construct a plasmid vector that contains a vaccinia virus protomer (p7.5, p11, etc.) and a marker gene (GFP, mcherry, etc.). I can find the sequences of the promoter. For example, the sequence of the p7.5 is

5'-TAAAAGTAGAAAATATATTCTAATTTATTGCAC-3’[J. Mol. Biol. (1989) 210, 749-769]

but what should be the length and the sequence of the marker gene?

Thank you in advance!

promoter • 326 views
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