Extract pre-mirna using sliding window.
Hello,
I want to extract the precursor of miRNA, and for this, I need to follow this step.
precursor sequences by taking a sliding window of about 100 nt (moving in increments of approximately 10 nt) from the region ~80 nt upstream of the beginning of the mature miRNA to ~80 nt downstream of the miRNA.
Please let me know how can i do this step. Should I use bedtools for this? I am confused kindly let me know.
• 1,123 views
•
link
updated
by
Ram
•
written
by
bioinfo223 •
•
written
by
bioinfo223 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Which locus should be considered the true ortholog after liftOver disagreement between chrUn and ch…
written by jhonatan.henrique •
-
Detect precursor but not mature miRNA in small RNA-seq data?
written by Rey •
-
Best practice to find break point in fusion genes
written by pablo
-
Question about Isoform expression (miRNA) data from TCGA
written by Vasu
-
counting the reads that map to a special window
written by Christleemans •
-
Sliding window in Trimmomatic and PrinSeq
written by seta
-
mature miRNA prediction from precursor sequences
written by micro32uvas •
-
sliding windows with or without overlap?
written by Yu
-
Precursor identification of mature miRNA
written by Prasad
-
A query on pre-miRNAs
written by The Last Word
For known ones you can use miRbase
Here is an article related to the discovery of novel miRNAs