align_and_estimate_abundance.pl
In align_and_estimate_abundance.pl script can we use both paired end and single end data at the same run? Or we need to execute them separately?
trinityrnaseq
expression-values
• 520 views
•
link
updated
by
Ram
•
written
by
Roy.anupama11 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Integration of paired and single-end SRA
written by rezaHi Can I conduct a genomic data analysis project using the integration of paired-end and single-end data for variant identification, as well as for detecting …
-
Using RSem of Trinity, align_and_estimate_abundance.pl script, all folders for sample/condition is …
written by Roy.anupama11 •Hi, Folders for all the samples are not being created, only 1/4 is created using align_and_estimate_abundance.pl script provided by Trinity, is giving output. What to …
-
Rsubread: Aligning multiple single and paired-end reads from multiple files (lanes)
written by schaeferchristina15 •Hello, I am new to bioinformatics and looking for some help. I have 27 files from an Illumina output. There are 4 paired end and …
-
Merge single end and paired end illumina raw sequences for SNP calling
written by prashantwaiker •Hello there, I am new to bioinformatic analyses and want to do SNP calling from Illumina sequences. I have the raw reads from the sequencing …
-
converting RF strand-specific mRNA sequencing data to FR stand-specific
written by upadhyay.maulik •Dear all, We have carried out stand-specific mRNA sequencing (using the Illumina paired end protocol) of five tissues. For four tissues, we used FR strand …
-
Why ATAC-seq/CUT&RUN need paired-end sequencing?
written by liyc.stjude •Same as the subject, why ATAC-seq/CUT&RUN need paired-end sequencing? Why not single-end? I can understand that Hi-C absolutely needs paired-end sequencing because otherwise it is …
-
Paired end reads merged to single end for mapping - should i allow multi-overlap similar to paired …
written by devikaparvathy •I am using featurecounts after mapping of my reads. I have a paired-end sequencing data but Only a low percentage of reads were mapped when …
-
Why does STAR filter out many paired end reads while they align fine as single end?
written by KDL •I noticed that STAR filtered out a lot of reads when they were supplied in paired end fastq files but kept both of them when …
-
Is There Any Advantage Of Paired End Sequencing For Chip-Seq?
written by Fidel<p>Hi,</p> <p>I hope that some of you may have a recommendation regarding the use of paired-end sequencing over single-end for ChIP-seq. In principle we expect …
-
Need Script Or Software To Remove Unpaired Reads From Paired End Reads
written by yangfangisok •<p>I want to use AMOScmp to analyze illumina paired end data. AMOScmp requires the same number of paired file to build .afg file. The original …