Using Mutsigcv When Mutation Data Is Not Sufficient
Dear all:
I have a problem when using MutsigCV.
I sequenced 20 paired tumor and normal tissues, and called somatic mutations including missense, nonsense, splicing and synoymous. And this is sufficient for MutsigCV, as I have synoymous mutation data.
Next, I wish to use the data from published paper, however, I found that there are no synoymous mutations there. Only missense, nonsense, splicing mutations are included. How can I integrate my data and published data when using MutsigCV? Thank you!
Li
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To which paper do you refer?
Thank you for your attention, Giovanni. The paper is Exome sequencing of liver fluke–associated cholangiocarcinoma. The paper do not contain synonymous mutations.