Is it the same for Fastq files?
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How to pool two fasta files of biological replicates in Linux?
'Two biological replicates of HG and LG reads were pooled together for a de novo transcriptome assembly." - how to pool two fasta files of RNa-seq in Linux?
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GenoMax
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shanjana.shawon •
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Yes. But if you have paired-end data from multiple lanes (or whatever you are trying to get into one file) you need to follow the same order for R1/R2 files when you cat the datafiles.
cat lane1_R1_fq.gz lane2_R1_fq.gz > R1.fq.gz
cat lane1_R2_fq.gz lane2_R2_fq.gz > R2.fq.gz
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