modified bases in oligo/primer?
Hi All,
Can someone remind me what the Asterix (*), such as those appearing at the end of the line, mean in an oligo such as:
CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTTCTACTATTCTTTCCCCTGCA*C*T*G*T
Best regards,
Yongde
• 720 views
•
link
1 answer
It usually stands for modified bases. I know them as PTO bonds when you order primers/oligos, but probably it is any modified base and you need to check the legend of the sequence provider.
• 0 views
•
link
Log in to answer this question.