Hello,

I have been attempting to run BUSCO on genomes using SBATCH, which is done offline. I keep running into the issue that larger genomes (mammals) get stuck at the metaeuk step and does not proceed. I have tried the following:

1. Run bacterial genome which worked.
2. Took a partial portion of the mammal genome (koala) and it ran successfully giving an output.

however, when i run the whole mammal genome which is about 2GB in size, it shows:

2022-11-10 12:50:59 INFO: Running 1 job(s) on metaeuk, starting at 11/10/2022 12:50:59

slurmstepd: error: JOB 71 CANCELLED AT 2022-11-10T13:50:30 DUE TO TIME LIMIT ***

My slurm script: (that worked for the partial genome of 200MB but doesn't for the whole genome of 2GB)

#!/bin/bash
#SBATCH --account=nn****k 
#SBATCH --job-name=Busco_mam
#SBATCH --time=2:0:0
#SBATCH --mem-per-cpu=10G
#SBATCH --ntasks=16 --cpus-per-task=4 --ntasks-per-node=4

set -o errexit  # Exit the script on any error
set -o nounset  # Treat any unset variables as an error
module --quiet purge  # Reset the modules to the system default

module load BUSCO/5.4.3-foss-2021b

cd /cluster/projects/nn****k/Busco_genomes/mammal_genomes

busco -i koala_genome.fa -l mammalia_odb10 -o ../koala_busco_output -m genome -c 24 --offline  --download_path  /cluster/projects/nn****k/Busco_genomes/busco_downloads

I am new to using clusters and slurm, i apologise if it is a trivial issue. Any advice would be appreciated. Thank you in advance!