qPCR primer specificity evaluation

Question

[trichter]

I have designed qPCR primer candidates which target a specific bacterial group.

I am now looking for a tool that can give me predicted amplicons in any bacterial group outside of my target group.

Sounds trivial, but i havent found a good option. Primer Blast does not have a exclusion criterion, but this would be exactly what i want:

> Search the entire database for possible amplicons EXCLUDING my target group.

Preferably with my own database of bacterial groups.

Thank you!

[jv]

Preferably with my own database of bacterial groups.

If you are planning to build your own database, one option would be to build the db without your target group thereby allowing you to "search the entire database for possible amplicons" in the absence of your target group sequences. Alternatively search your whole db and filter out hits for your target group.