Hi everyone, I am using psiblast (Position-Specific Iterated BLAST 2.13.0+) to derive a PSSM file from multiple alignment file downloaded from the NCBI official website, but I always get this error:

BLAST query error: CAlnReader::GetSeqEntry(): Seq_entry is not available until after Read()

The code I used as below:

psiblast -in_msa sample_msa.txt -db nr  -out out.txt

the sample_msa.txt is here:

26SPS9_Hs IHAAEEKDWKTAYSYFYEAFEGYdsidspkaitslkymllckimlntpedvqalvsgkla
F57B9_Ce LHAADEKDFKTAFSYFYEAFEGYdsvdekvsaltalkymllckvmldlpdevnsllsakl
YDL097c_Sc ILHCEDKDYKTAFSYFFESFESYhnltthnsyekacqvlkymllskimlnliddvkniln
YMJ5_Ce LYSAEERDYKTSFSYFYEAFEGFasigdkinatsalkymilckimlneteqlagllaake

Does anybody know the problem?