Hi,
I'm trying to download and build a costumed reference and when I try doing the first step here https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/6.0/using/tutorial_mr
which is (wget ftp://ftp.ensembl.org/pub/release-97/fasta/macaca_mulatta/dna/Macaca_mulatta.Mmul_8.0.1.dna.toplevel.fa.gz) I keep getting this error message Error in server response, closing control connection. Retrying. what is the problem? and how to fix it?
Thank you!
1 answer
It's a problem with your or your institution's firewall. When you use the FTP protocol, wget will by default use active FTP and open a second port for the actual data transfer (usually using ports 20 and 21).
Either add the --passive-ftp flag to your command or just use http:// instead of ftp:// for downloading:
wget http://ftp.ensembl.org/pub/release-97/fasta/macaca_mulatta/dna/Macaca_mulatta.Mmul_8.0.1.dna_sm.toplevel.fa.gz
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Ensembl FTP site was not working yesterday (and perhaps until some point today). It is working now so unless it is a firewall issue at your local institution @Matthias' command should work now.
Yes, it is working now