Hello! I have a strange situation, I can't figure out what's wrong. I would be very grateful if someone could give me an idea. Started a mass spectrometer and decided to test on known proteins (insuline from GEROPHARM, cytochrome C). Nothing works.

MS1: After Peak Picking in msconvert output files in mgf format are empty. If we upload raw files to maxquant or ProteomeDiscoverer, they don't find anything. At the same time, the chromatograms are good and the peaks are clear .

MS2: Сytochrome C is well defined. However, insulinIs not defined even with a small probability by either instrument.

The problem is not storage samples or enzyme processing. There is no problem in the structure of the raw files either.