RNA-Seq quantification methods
Hi all,
In RNA-Seq, there are two ways of quantification:
- using a reference genome
- directly on the transcriptome
I read about that but I still don't understand the second way. Would anyone can give an easy-to-understand material or you can explain it here?
Thank you so much!
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what exactly you don't understand - the methodological details or the general idea?
The general idea. It is quite hard for me to understand this if just Googling.
there are many threads about this in biostars - for example take a look here Alignment and mapping or here Could you explain the difference between STAR, KALLISTO, SALMON etc. to experimental Biologist/non-bioinformatician