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RnBeads mRatBN7.2 annotation package dbSNP location + chromosome

Hi All,

I have been analysing some rat data with the default rn5 package and am now trying to upgrade to a more recent reference genome.

I am following the instructions on this page https://rnbeads.org/data/RnBeadsAnnotationCreator.pdf

I have two main questions regarding this.

My main issue is that the most recent dbSNP data for rats is in a different ftp site, rather than the ncbi url in the documentation they are in an ebi server. how would I best access these to suit RnBeads. Here is the location : https://ftp.ebi.ac.uk/pub/databases/eva/rs_releases/release_3/by_species/rattus_norvegicus/mRatBN7.2/

Secondly When selecting chromosomes where can I find the annotation used in a specific reference assembly for a bioconductor package such as rn7? The rat genome has 1:20, X ,Y ; so would it be:

CHROMOSOMES -> c(1:20, X, Y)?

rn7 rrbs rnbeads

1 answer

I have solved the issue by downloading the current ids vcf file locally and using the recommended 1:20, X.

I just replaced the vcf.files with the location of the downloaded file.

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