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sequence error

Hi,

What kind of base sequence errors could happen more frequently at MiSeq? I found a document saying G > A and C > T could occur more, but why?

Thank you.

ngs miseq
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written by maricom • maricom

1 answer

Those base mutations are called transitions. Purine-> Purine or Pyrimidine -> Pyrimidine. Transversions are when a purine is mutated to pyrimidine or vice-versa. Transitions are more common in nature and in PCR. Some related links:

  1. https://en.wikipedia.org/wiki/Transversion
  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720931/
  3. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5026502/
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written by mark.ziemann mark.ziemann

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