Differential expression pattern of known miRNAs
Hi all, I am naive in sRNA-seq data analysis. I mapped my sRNA-seq data to mature and precursor plant miRNAs from miRBase. Now I want to study the expression patterns of these miRNAs across different samples.
- For some miRNAs, only precursors have been identified. Can I use their mature sequences instead of precursor for differential expression analysis?
- only mature miRNAs are used for differential expression analysis? as for some miRNAs, both mature and precursor were detecetd.
- In my list, for example, the sequence of aof-miR167a and ata-miR167e-5p is the same. Could I include all miRNAs in the final file for differential analysis? or I should only use one of them?
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
How to run quantifier from miRDeep2 (miRNA seqs from MirGeneDB)
written by Apex92 •
-
Merging csv files with specific headers
written by windsur •
-
Detect precursor but not mature miRNA in small RNA-seq data?
written by Rey •
-
What does the part "mature miRBase miRNAs not detected by miRDeep2" actually mean in a miRDeep2 ana…
written by Debasish •
-
How to collapse redundant miRNAs predicted by miRDeep2?
written by 2822462298 •
-
convert hsa miRBase gff3 into gtf
written by anna.coenenstass •
-
categorization of miRNA and miRNA star
written by Sam
-
Duplicate miRNAs with different read counts in miRDeep2
written by PAn •
-
miRNA sequencing question
written by delgrodel •
-
miRNA annotation from miRBase 21
written by bioinforupesh2009.au •
