Clustering Seurat SCTransformed data with only count data
Hi,
I downloaded some scRNA seq data from GEO. It is in the form of expression matrixes where the counts have been normalized with SCTransform already. Since the data is just the counts matrix and not a seurat object when I try to run PCA to cluster it I get the error that i need to run FindVariableFeatures() and ScaleData(). I believe these steps are usually built in to the SCTransform process so I'm worried that doing it again will mess up the data. Is there a way around this?
My goal is to cluster the cells in this dataset so I can identify cell types.
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