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Percentage of mitochondoria in single nuclei single cellRNASeq

Hi All

Are these percentages of mitcohondoria seems normal for single nuclei/Single Cell RNASeq? What is a normal rate? Thanks

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cell mitochondoria nuclei single rnaseq

1 answer

I recommend you this paper : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8599307/ Particularly : "mtDNA% ranges from 5% or less in tissues with low energy requirements up to ∼30% in the heart due to the high energy demand of cardiomyocytes" So it depends on your tissue but your percentages are in the acceptable range.

Thank you Basti very much. But this is even if it is single nuclei? The paper talks about single cell? Thanks

You're right and I don't see any paper that studied the subject in greater depth, but I believe the percentage should be lower. An exemple here in Fig 2D : https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-021-00941-8 "the percentage of mitochondrial genes sequenced in the nucleus-containing droplets was lower than the cell-containing particles (2.7% and 4.2%, respectively)" and they filtered out nuclei with >20% mitochondrial gene counts.

Got it. Thank you very much Basti.

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