identification of agilent micro array non coding RNA id
Dear all, I tried various methods to identify the ensembl id or gene id for non-coding micro array agilent id-ASHGV40053163.but i couldnt . It will be very useful for my research work. ncbi geo dataset-GSE124352 Please help me identify this non coding RNA. Thank you. Dr. Pradeep Kumar, MDS
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If you look at the platform https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL21827 table that ID has the following sequence from hg19 build. It is denoted as non-coding.
CCTCAAGTGCAAAATGTATAAACTCATCATAGCAATAAATTCCCGCATCATGCATTTCAA
If you blat with the sequence at UCSC you get the following hits.
ACTIONS QUERY SCORE START END QSIZE IDENTITY CHROM STRAND START END SPAN
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browser details YourSeq 60 1 60 60 100.0% chr9 + 98257598 98257657 60
browser details YourSeq 21 18 38 60 100.0% chr5 - 21130605 21130625 21
browser details YourSeq 20 11 30 60 100.0% chr3 + 63194722 63194741 20
First one appears to be full size and that would be your best bet. It seems to be in the intron of PTCH1 gene.
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