This is a test version of Biostars. For the public version, visit https://www.biostars.org.
identification of agilent micro array non coding RNA id

Dear all, I tried various methods to identify the ensembl id or gene id for non-coding micro array agilent id-ASHGV40053163.but i couldnt . It will be very useful for my research work. ncbi geo dataset-GSE124352 Please help me identify this non coding RNA. Thank you. Dr. Pradeep Kumar, MDS

micro id conversion array

1 answer

If you look at the platform https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GPL21827 table that ID has the following sequence from hg19 build. It is denoted as non-coding.

CCTCAAGTGCAAAATGTATAAACTCATCATAGCAATAAATTCCCGCATCATGCATTTCAA

If you blat with the sequence at UCSC you get the following hits.

   ACTIONS      QUERY   SCORE START   END QSIZE IDENTITY  CHROM  STRAND  START       END   SPAN
-----------------------------------------------------------------------------------------------
browser details YourSeq    60     1    60    60   100.0%  chr9   +    98257598  98257657     60
browser details YourSeq    21    18    38    60   100.0%  chr5   -    21130605  21130625     21
browser details YourSeq    20    11    30    60   100.0%  chr3   +    63194722  63194741     20

First one appears to be full size and that would be your best bet. It seems to be in the intron of PTCH1 gene.

Log in to answer this question.