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Strange peak in gene coverage plot (MultiQC)

Hi everyone,

I have encountered an issue with some pilot data for an RNA-seq project using low-input SMARTer total stranded RNA-seq (tissue collected from non-mammalian brains using LCM). I received the MultiQC report, and the gene coverage profile has a weird peak consistently across all samples. None of my colleagues know what the cause might be. Any ideas?

Would appreciate any advice...

Thanks!

Gene Coverage Profile

qc genebody_coverage rna-seq pico smarter
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updated by GenoMax GenoMax • written by Fanny • Fanny

Is the sequence in that region low complexity? Perhaps the way these alignments were done reads multimapped in this region more than one time?

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written by GenoMax GenoMax

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