Hello Everyone,

I dont have a lot of experience with the usage of Guppy besides the information u can get on the community protocol page and other github sides.

I am currently trying the Tool for the first time and i am encountering a little problem.

When i try to do an alignment with the human ref Genom in the Base_caller the process always gets killed.

I am trying to get the information about modified bases in some samples in form of bam files.

Here is the configuration i am using currently:

guppy_basecaller \ --config dna_r9.4.1_450bps_modbases_5mc_hac.cfg \ -x 'auto' \ --num_callers 1 \ --chunk_size 1000 \ --chunks_per_runner 48 \ --gpu_runners_per_device 2 \ --bam_out --recursive --compress \ --align_ref /path to ref/Homo_sapiens.GRCh38.dna.alt.fa \ -i /path to input -s /save path

It always starts the process and then the only output i get in the terminal is "killed" after looking more into the Error Message i get an cgroup mem Limit Error. I use 12G for this analysis i would assume i need more mem to do an Alignment with the human_ref Genome.

It would be a great help if someone could help me. Thanks a lot!