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ERROR: This has possibly been a NUCmer run failure, please investigate ERROR: NoneType: None

Hi; i am trying to run pyani and it gives this error-

average_nucleotide_identity.py -i
/home/bvs/neelam/genome_clust/Clust_13 -o AND/AMR_ANIm_output -m ANIm
-g --worker 40 --maxmatch

WARNING: At least one NUCmer comparison failed. ANIm may fail.
WARNING: Total alignment length reported in AND/AMR_ANIm_output/nucmer_output/P_aeruginosa_E90_vs_P_aeruginosa_P9W.filter is zero!
WARNING: Total alignment length reported in AND/AMR_ANIm_output/nucmer_output/P_aeruginosa_E90_vs_P_aeruginosa_AR_458.filter is zero!

ERROR: This has possibly been a NUCmer run failure, please investigate
ERROR: NoneType: None

Thank you!

pyani

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