Hi everyone!

Im trying to deal with rare variants for the first time and I need to use RIVER (RNA-Informed Variant Effect on Regulation) package. I completely don't understand how to create correct input file. I can use data from dbGAP. Maybe someone can share experience of using RIVER (creating input)? As I undersood, I should create matrix with features, N2 pairs and Z-scores. Which raw file format I need? How N2 pairs can be calculated? Any recomendation will be really helpfull.