Hello, I have been using txImport (which ATPoint recommended) to convert my transcript level RNA files to gene level files. (basically to add up all the transcripts to get one TPM value per gene). When I add up genes manually in excel, it doesn't always match tximport's TPM value. Here is a comparison of the TPMs:

Gene- [Excel] vs [TxImport Result]

• Aanat - 0 vs 0
• Aatk - 0.29 vs 0.17
• Abca1 - 3.28 vs 3.28

• Abca4 - 0.25 vs. 0.22

• Abca2 - 3.2 vs 2.75

• Abcb7 - 0.95 vs 0.95

Manual Count in Excel:

. ___

TxImport Result:

I wanted to ask if this is an error or usual thing for tximport. Does it sometimes skip some transcripts?