I am carrying out taxonomic classification of some metagenomic WGS samples (sequenced using Illumina NovaSeq and NexteraPE adaper libraries) using Kraken2 and I have a …
I have a discrepancy between STAR and other software I cannot reconcile. I'm working on RNA-seq data from *B. subtilis*. The sequencing facility is adamant …
Hi, I've been running Kraken2 and KrakenUniq (using the --report-minimizer-data flag) on my metagenomic samples. When I sum up the per level read counts for …
Hello, I'm processing a bulk RNa-seq dataset which was aligned using STAR, counted using Salmon. The quant.sf files were then processed by tximport into a …
Hi, I'm performing data analysis of samples of which the RNA has been sequenced. I've gotten pretty significant differences in total gene counts of samples. …