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demultiplexing error

Hi,

I am using the mrDNA fastq splitter https://www.mrdnalab.com/mrdnafreesoftware/fastq-splitter.html to demultiplex reads. It requires the R1, R2 and mapping file as inputs. Has anyone encountered this problem where it just stops halfway and gives the following error:

'Error writing the result text files. The process cannot access the file E:\Data\S1_R1.fastq because it is being used by another process'

I have tried from the C drive and D drive and i still get the same error and have also made sure that no files linked to this are running in the background

demultiplexing

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