NOISeq, GFOLD or EdgeR for identifying DEG with no replicate?
Need help!
I have 4 sequencing libraries corresponding to different developmental stages, each library was constructed from total RNA extracted from pulled embryonic tissues. So the sequencing data do come from several embryos. But when conducting bioinformatic analysis, I'm not sure whether I should use NOISeq, GFOLD or EdgeR? I'm new in bioinformatics.
May I have your suggestion? Many thanks!
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