Hi everyone, This is my first post in Biostars so please excuse me for mistakes.

I am quite new with analysing nucleid acid sequence data in R. Recently trying to predict all possible translation products from DNA sequence. Therefore using systemPipeR::predORF to predict ORFs and using Biostrings::translate to translate. Things work ok but am not quite able to understand the effect of the setting longest_disjoint = TRUE/FALSE within predORF.

Documentation says: longest_disjoint: If set to TRUE and n='all', the results will be subsetted to non-overlapping ORF set containing longest ORF.

I do want to set it to True, but when I do that some of the longest ORFs from when I set it to False are missing when I set it to true.

Any help from the folks who have used it. Cheers and many thanks