Good day.
I did an RNAseq, which I assembled by Trinity, then I did a BlastN. BlastN results filtered and organized in a .xlsx file What software can I use to make a taxonomic classification from this file?
# query subject_id subject_def pident align_length qstart qend sstart send bitscore e_value
TRINITY_DN91_c0_g1_i4 gi|1218258742|ref|XM_021852686.1| [Candida] intermedia strain CBS 141442 genome assembly, chromosome: I 117 142 1018 1157 247055 246916 121.15 4.79e-22
TRINITY_DN91_c0_g1_i4 gi|1218258740|ref|XM_021852685.1| [Candida] intermedia strain CBS 141442 genome assembly, chromosome: I 117 142 1018 1157 247055 246916 121.15 4.79e-22
TRINITY_DN91_c0_g1_i4 gi|122937760|gb|EF173372.1| [Enterobacter] lignolyticus strain G5 chromosome, complete genome 420 434 90 523 110398 110831 725.01 0.00e+00
TRINITY_DN91_c0_g1_i4 gi|122937760|gb|EF173372.1| [Enterobacter] lignolyticus strain G5 chromosome, complete genome 420 434 90 523 208214 208647 725.01 0.00e+00
•
written
by
weimabri •
A tool like MEGAN can combine the various BLAST hits into hierarchical classification model
The result of the BlastN, I have it in this format. BLASTN_NTAgo2021_Trinity_clean_Com06.Trinity.fasta.xml.tab
But the MEGAN6 does not identify it as a BLAST format, Do you know of a way to change it to a format that identifies Megan?
It needs to have certain columns, look up what those are. There are also various other input formats that you may be able to change your data into.
Log in to answer this question.