How to calculate fold change with GSE data
Hello everyone,
I'm performing a single cell RNA seq analysis with Bioconductor (Seurat precisely) to cluster immune cells present in Brown Adipose Tissue (BAT) of Mus musculus. I started from data of the dataset GSE177635. I've just clustered populations and now I want to calculate the Fold Change of a specific population (macrophage population number six) to visualise the differential expression between genes expressed in wild type (control cells) and genes expressed in treated cells. I'm wondering, how to calculate the Fold Change starting from this clustering?
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