Thank you so much
Hello, a little bit about myself. I'm a first year bioinformatic grad student who somehow got himself into a mock metagenomic project (there will be an actual project in half a year). I have no prior experience with metagenomics side and there are no courses being offered in my university that covers the basic of metagenomic.
For the project, I am expected to 1) find and download human metagenomic data (raw reads), 2) perform taxonomic assignment (taxonomic profiling, etc), and 3) perform comparative metagenomic is possible.
I somehow managed to download files (in fastq.gz format) from the human microbiome project database. I vaguely know I should use software such as Kraken but I honestly have no clue (and no one to ask in rl) who should I even begin. If anyone can point me towards the right direction or leave hints, it will be a life saver.
1 answer
Read some papers
- 2017 Shotgun metagenomics, from sampling to analysis
- 2017 Critical Assessment of Metagenome Interpretation—a benchmark of metagenomics software
- 2019 Benchmarking Metagenomics Tools for Taxonomic Classification.pdf
- 2020 LEMMI: a continuous benchmarking platform for metagenomics classifiers
- 2021 Critical Assessment of Metagenome Interpretation - the second round of challenges
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