Hello,

I have imported sample SRA to my local machine, then I have converted the SRA to fastq using sra-toolkit from ncbi. As a next step, I would like to perform quality trimming using sicke: https://github.com/najoshi/sickle The problem is that i don't know which quality type format to use (solexa, illumina or sanger). If I understand correctly, fastq files should have some kind of normalized format, (https://www.ncbi.nlm.nih.gov/sra/docs/sra-data-formats/ ), however I dont know which one is it.

Does this normalized format means that all fastq files from different bioprojects has the same quality scores? If not, how do I determine the correct format?

Thank you in advance, Pawel