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rRNA Contamination in the RNA-seq library

I have to run CIRI on several fq files for the identification of circular RNA but realize that there is a small percentage of rRNA contaminants within them. know We did not remove the contamination before library construction earlier as protocol. Now that I have run hisat2 on files and CIRI on .sam for circular RNA identification. Is there a way to remove the contaminants? Thanks!

transcriptme ngs cirrna rrna

1 answer

I'm not 100% clear on your objectives for filtering your data, but bbduk from BBMap can be used to remove reads from a fastq file that match to a set of user defined sequences (in your case, rRNA sequences).

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