Is there a tool available to check a Tm for a DNA fragment or sequence?
Is there a tool available to check a Tm for a DNA fragment or sequence?
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mmghaffar29 •
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mmghaffar29 •
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There's multiple software out there. I'd suggest this, written in R. https://aravind-j.github.io/rmelting/reference/melting.html
Based on SantaLucia [1] (as mostly of the software out there)
If you are doing this manually then a lot of wet lab people use: https://www.thermofisher.com/ch/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html
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