Trimmomatics Error: M1 Mac
Was using trimmomatcs for ERR1465930 and received an error
Multiple cores found: Using 4 threads
Exception in thread "main" java.lang.RuntimeException: Unknown trimmer: LEADING
at org.usadellab.trimmomatic.trim.TrimmerFactory.makeTrimmer(TrimmerFactory.java:73)
at org.usadellab.trimmomatic.Trimmomatic.createTrimmers(Trimmomatic.java:59)
at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:552)
at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)"
I used the code
trimmomatic PE ERR1465930_2.fastq.gz ERR1465930_1.fastq.gz \ ERR1465930_1_paired.fastq.gz ERR1465930_1_unpaired.fastq.gz \ ERR1465930_2_paired.fastq.gz ERR1465930_2_unpaired.fastq.gz \ LEADING:10 TRAILING:10 SLIDINGWINDOW:5:20 MINLEN:250
What exactly was wrong?
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Not sure if you had the
\in your actual command or you put them in while trying to format your command line. In either case they don't need to be there. Check the order of your file names.You also appear to be specifying R2 file before R1.