I'm trying to align PE fastq files using 'bwa mem' and in the command I want to add the read group information so that it appears in the output .sam file

Note: I'm using jupyter notebook here is my piece of code

R1 = os.path.join(rootdir, "001_S001_L001_R1_001.fastq") 
R2 = os.path.join(rootdir, '001_S001_L001_R2_001.fastq')

SM = os.path.basename(R1).split('_')[0]                     ## Sample ID
LB = '_'.join(os.path.basename(R1).split('_')[0:2])         ## Library ID
PL="Illumina"                                               ##platform (e.g. illumina, solid)

with open(R1) as f:
    firstline = f.readline()
    RGID = '_'.join(firstline.replace(':','_').split('_')[0:4])

PU= RGID + '_' + LB                                                  ## Platform unit
index_file = '/home/phoebemagdy/workdir/PhD/Trial_Code/Ecto_Data/BWA_alignment/BWA_Index/hg19.fa'

!bwa mem -t 2 -M -R "@RG\tID:{}\tSM:{}\tPL:{}\tLB:{}\tPU:{}".format (RGID, SM, PL, LB, PU) {index_file} {R1} {R2}>  First_patient.sam

I always get the following error : /bin/bash: -c: line 0: syntax error near unexpected token (' /bin/bash: -c: line 0:bwa mem -t 2 -M -R "@RG\tID:{}\tSM:{}\tPL:{}\tLB:{}\tPU:{}".format (RGID, SM, PL, LB, PU) {index_file} {R1} {R2}> First_patient.sam' 1

I'll be grateful for any suggestions to help fixing this error...!