Is it possible to infer from miRNA-seq fastq data which species it is obtained from?
I am analyzing human miRNA-seq data. I have done mapping with HISAT2 or STAR, but I am having trouble because the mapping rate is very low, 10-30%.
I am even thinking that maybe this is not human RNA, but RNA data from other organisms. So, I would like to ask, is it possible to estimate from the fastq data of miRNA-seq which species it is obtained from?
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Has it been trimmed properly and what reference did you align against?
also a quick comparison with annotated human miRNAs in miRbase can verify the origin
Sorry for the lack of study, but how do I do that?
I'm sorry for late reply. Of cource, I trimmed properly. I confrimed that I used GRCh38 as a reference genome.