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Quantifying expression levels of IsoSeq reads with Kallisto

Hi, I have IsoSeq read data and I would like to quantify expression levels of novel isoforms (which I integrate to the reference genome) using Kallisto. Any idea of specific Kallisto usage for this case?

isoseq kallisto expression

1 answer

This is long-read sequencing data. Currently, most standard tools (including kallisto) are only suitable for short read sequencing data.

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