RNA-seq total read count & nucleotide relationship
To calculate the total number of reads per sample, is it correct to sum up the reads in all flowcells & lanes, and also sum up the reads for Read1 & Read2 when using paired-end sequencing?
Furthermore, unlike in DNA-Seq, the nucleotide content per cycle of A&T and G&C does not have to be equal because mRNA is single-stranded.
Are these statements correct?
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