Best practice to pool samples for sequencing?
Hello, it’s my first time having a library sequenced. I have 96 samples with varying concentrations. We are supposed to deliver a 4nm pool to the sequencing facility. My question is; what is the best way to pool all the samples?
Does it make sense to make an equimolar pool first in ng/ul and then diluting this to the required nm? Or is it better to immediately convert all concentrations from ng/ul to nm and then calculate a pool?
I am a little bit confused …
Thanks!
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This is not bioinformatics-related and as such off-topic. If you collaborate with a sequencing facility I recommend to always first talk to them, as they're experienced. I am sure they can give you some best practices. Facilities we worked with have always been very helpful and friendly when asked for help.