Hi all,

So I am currently attempting to use Enhanced volcano to visualise a set of RNA-seq data, but when I run the below script, I get back the error message below. Does anyone know what the problem is - I can't see anything wrong in the code.

Error in EnhancedVolcano(RV16_byweights, lab = RV16_byweights$SYMBOL,  : 
  unused arguments (pCutoffCol = "adj.P.Val", max.overlaps = Inf, maxoverlapsConnectors = 50)

My File is labelled - RV16_byweights

Head of file:

My code:

EnhancedVolcano(RV16_byweights, #dataframe
            lab = RV16_byweights$SYMBOL, #gene symbol for labels
            x='logFC', y='P.Value', #values for axis
            title='RV16 Volcano plot', 
            subtitle='With Quality Weights',
            pCutoffCol = 'adj.P.Val', #use adjusted p value for cutoff value 
            pCutoff = 0.05, FCcutoff=0.5, #cutoff lines for graph 
            ylim = c(0, 10), #axis limits
            xlim = c(-2, 4),
            pointSize=2, labSize=3, #point and label size
            colCustom = keyvals2, #custom colours set up above
            colAlpha = 5,
            legendPosition = 'bottom',
            legendLabSize = 10,
            legendIconSize = 5,
            axisLabSize = 10,
            xlab = 'LogFC', 
            drawConnectors = TRUE, #lines from labels to dots
            widthConnectors = 0.25, #width of connectors
            max.overlaps = 20,
            maxoverlapsConnectors = 50, #max number of overlaps allowed
            colConnectors = 'black', 
            boxedLabels = FALSE, #box around labels 
            arrowheads = FALSE, 
            gridlines.major = TRUE,
            gridlines.minor = FALSE,
            border = 'full',
            borderWidth = 1,
            borderColour = 'black',
            )